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Rheb-GTP

26910

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Rheb-GTP

Cat. #:   26910
Size:  30 µL
Gene Symbol:  RHEB
Description:   Anti-Rheb-GTP Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).
Immunogen:   Recombinant full-length protein of active Rheb.
Applications:   IP, IHC, IF
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgM
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze/thaw cycles
Immunoprecipitation/Western blot:   Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).
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