Ras-GTP

26909

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Ras-GTP

Cat. #: 26909

Size: 30 µL

Gene Symbol: RAS

Description: Anti-Ras-GTP Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Immunogen: Recombinant full length protein of active H-Ras

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

IP/WB Analysis of Active Ras Protein.Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Ras-GTP monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # 26014).

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Ras-GTP

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