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Sar1-GTP

26916

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Sar1-GTP

Cat. #::  26916
Size:  30 µL
Gene Symbol:  SAR1
Description:   Anti-Sar1-GTP Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.
Immunogen:   Recombinant full length protein of active Sar1a.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).
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