Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Arf6-GTP
Cat. #: 26918 |
Size: 30 µL |
Gene Symbol: ARF6 |
Description: Anti-Arf6-GTP Monoclonal Antibody |
Background: ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis. |
Immunogen: Recombinant full length protein of active Arf6. |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Arf6-GTP monoclonal antibody recognizes active Arf6 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: |
Arf6 activation assay. Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase