Anti-EML4 ALK Antibody

Cat. #:   26242

Gene Symbol:  EML4-ALK

Description:   Anti-EML4 ALK Mouse Monoclonal Antibody

Background:   EML4-ALK is a fusion-type protein kinase that can be found in many tumors, such as anaplastic large cell lymphoma, inflammatory myofibroblastic tumor, neuroblastoma and NSCLC, and so on. It is generated as a result of a small inversion within the short arm of human chromosome 2. EML4-ALK protein often promotes and maintains the malignant behavior of the cancer cells by activating the MAPK, PI3K/AKT and JAK/STAT3 pathways.

Immunogen:   A synthetic peptide from the internal region of EML4-ALK of human origin.

Applications:   ELISA, WB, IHC

Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host Species:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:

Purity:   Purified from ascites

Preservative: No

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes T41A mutant, but not wild type EML4-ALK of vertebrates.

Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:   Western blot analysis of recombinant EML4-ALK and wild type proteins. Purified His-tagged EML4-ALK protein (lane 3), corresponding wild type EML4 protein (lane1) and wild type ALK protein (lane2) were blotted with Anti-EML4 ALK monoclonal antibody (Cat. #26242). Immunofluorescence:   Immunofluorescence of cells expressing EML4-ALK proteins with Anti-EML4 ALK antibody. HEK293T cells were transfected with pCDNA3-GFP-EML4 (WT) plasmid (left column) or pCDNA3-GFP-ALK (WT) plasmid (middle column) or pCDNA3-GFP-EML4-ALK plasmid (right column), then fixed and stained with Anti-EML4 ALK monoclonal antibody (Cat. #26242).

No data available