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Cdc42 pAb

21010

Size:100 μl Price:$ 219 Brand:NewEast Place of Origin:USA Immunogen:

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Cdc42 Polyclonal Antibody

Cat. #:  21010
Gene Symbol:  CDC42
Description:   Anti-Cdc42 Rabbit Polyclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen:   A synthetic peptide from a unique region of Cdc42
Applications:    ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:100-1:5000
WB: 1:100-1:2000
IHC: 1:50-100
Concentration:   0.2 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Cdc42 antibody recognizes Cdc42 from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of Cdc42 proteins.

MEF whole-cell lysate was used for western blot with the polyclonal Anti-Cdc42 antibody (cat. #21010).

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