Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26038 |
| Gene Symbol: Ras(G13D) |
| Description: Anti-Ras(G13D) Mouse Monoclonal Antibody |
| Background: Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% glycerol |
| Species Reactivity: Anti-Ras(G13D) monoclonal antibody recognizes RasG13D proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant Ras WT and G13D proteins with anti-Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti-Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021).
Immunofluorescence:
Immunofluorescence of cells expressing RAS proteins with Anti-Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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