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PIK3CA(E545K) pAb

21145

Size:100 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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PIK3CA(E545K) Polyclonal Antibody
Cat. #:  21145
Size:  100 µL
Gene Symbol:  PIK3CA(E545K)
Description:   Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody
Background:   Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers.
Immunogen:   A synthetic peptide from the internal region of PIK3CA(E545K) of human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:    ELISA: 1:100-1:5000 WB: 1:100-1:1000 IHC: 1:10-1:100
Concentration:   0.2 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Purity:   Purified from serum
Preservative:  No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Western blot:  
Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with Anti-PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145).
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