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Human KGF (C-6His) Protein

12045

Size:50 μg Price:$ 525 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:     12045
Product Name:   Human KGF (C-6His) Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   Fibroblast growth factor 7;FGF-7;Heparin-binding growth factor 7;HBGF-7;Keratinocyte growth factor;FGF7
Target:    KGF
UNIPROT ID:    P21781
Description:    Recombinant Human Fibroblast Growth Factor 7/Keratinocyte growth factor is produced by our Mammalian expression system and the target gene encoding Cys32-Thr194 is expressed with a 6His tag at the C-terminus.
Background:     Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.
Species/Host:    HEK293
Molecular Weight:    20 KDa
Molecular Characterization:    Not available
Purity:    Greater than 95% as determined by reducing SDS-PAGE.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Greater than 95% as determined by reducing SDS-PAGE.

Figure 2. Measured in a cell proliferation assay using HaCaT cells. The ED50 for this effect is 10.94 ng/ml.
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