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TDGF1 Rabbit pAb

222419

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S222419
Product Name:   Anti-TDGF1 Rabbit Polyclonal Antibody
Synonyms:   CR; CR-1; CRGF; CRIPTO
UNIPROT ID:   P13385 (Gene Accession - NP_003203 )
Background:    This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human TDGF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Stem Cells, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222419(TDGF1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222419(Anti-TDGF1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 222419(TDGF1 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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