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NCF1 Rabbit pAb

222434

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S222434
Product Name:   Anti-NCF1 Rabbit Polyclonal Antibody
Synonyms:   CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
UNIPROT ID:   P14598 (Gene Accession - NP_000256 )
Background:    The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Immunogen:    Synthetic peptide of human NCF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222434(NCF1 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222434(Anti-NCF1 Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 222434(Anti-NCF1 Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D264603(Anti-NCF1 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Raji, RAMOS cell, Mouse brain tissue lysates;
Primary antibody: 222434(NCF1 Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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