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Rad21 Rabbit mAb

263520

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263520
Product Name:   Anti-Rad21 Rabbit Monoclonal Antibody
Synonyms:   RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog
UNIPROT ID:   O60216
Background:    Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis.
Immunogen:    A synthetic peptide of human Rad21
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-5I0
MW:    Calculated MW: 72 kDa; Observed MW: 130 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Hamster
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Rad21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Rad21 (green) in Hela using Rad21 antibody,and DAPI(blue).

Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.
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