Polyclonal Antibody Production Service - NewEast Biosciences - GTPase, Oncogene and Bioactive Protein

Polyclonal Antibody Production Service

Custom Polyclonal Antibody Production

We has more than ten years of experience in antibody preparation and is well versed in the key aspects of polyclonal antibody preparation. The host for the antibody can be rabbits, mice, rats, guinea pigs, hamsters, goats, chickens or other species. The titer of polyclonal antibodies can reach more than 1:10000. Whether the antigen is recombinant proteins, peptides and virus particles, we can provide you with a complete set of solutions from target design, antigen preparation to production, purification and quality testing, ensuring that the prepared antibodies can be effectively used in project research.

Highlights

Free Antigen Design – Based on experience, data, and characteristics of your desired target.
Wider Choice of Host Animals – Choose from SPF (specific-pathogen-free) host animals, including rabbit, mouse, rat, and guinea pig.
High Specificity – Antibodies are purified by Protein A and antigen affinity chromatography. When needed, we can provide cross-purification to obtain a more specific antibody.
Guaranteed Result – 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted at 1:6000.

Process Workflow

Step	Details	Timeline
Antigen design and preparation	Recombinant protein production and peptide synthesis and conjugation	Week 1-3
Negative serum collection	Collect 3-5 ml control serum before immunization	Week 2-3
First Immunization	Subcutaneous multipoint immunization	Week 2-3
First Booster	Subcutaneous multipoint	Week 4-5
Second Booster	Subcutaneous multipoint	Week 6-7
Blood Test	50 µL of serum was collected from each mouse, and the titer was detected by ELISA	Weeks 7-8
Third Booster	Subcutaneous multipoint immunization	Weeks 9-10
Final blood collection	20 ml serum was collected from each rabbit, and the titer was detected by ELISA	12 Week
Shock Immunity	Ear vein immunity	Weeks 11-12
Final blood collection	Collect 40-50 ml of blood from each rabbit	Week 12-13
Antiserum Purification	Choose protein A or antigen affinity purification according to customer requirements	Week 14-15
Project Delivery	Antibody delivery and project production report	Weeks 15-16

Remark

Host: New Zealand White Rabbit
Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.

Deliverables

200 μL pre-immune negative serum (50 μL for mice);
More than 1 mg of antigens or polypeptides prepared by Primetal;
3-5 mg of antigen affinity-purified polyclonal antibody (concentration > 1 mg/mL);

Custom Polyclonal Antibody Production

Highlights

Process Workflow

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