Monoclonal antibody customization service

Mouse Monoclonal Antibody Service

Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.

Monoclonal antibodies are a class of antibodies that are produced by hybridoma cells by cell fusion technology and can bind to specific antigenic epitopes. Monoclonal antibodies have the characteristics of batch stability, high specificity and easy large-scale preparation, so they are widely used in biological research, in vitro diagnosis and drug development. According to the different needs of customers, we customize different solutions according to the type, quantity and purity of their antigens; through HAT screening and multiple subcloning screening to ensure 100% purity of cell lines; we use antibody affinity purification to ensure high antibody purity, Whether you use it downstream for diagnostic testing or drug research, its effectiveness is guaranteed.

Step	Details	Timeline
Antigen design and preparation	Recombinant protein production and peptide synthesis and conjugation	1-3 Weeks
First Immunization	Subcutaneous multipoint immunization	4 Week
First Booster	Subcutaneous multipoint immunization	7 Weeks
Second Booster	Subcutaneous multipoint immunization	9 Weeks
Blood Test	50 µL of serum was collected from each mouse, and the titer was detected by ELISA	10 Week
Third Booster	Subcutaneous multipoint immunization	11 Week
Shock Immunity	Intraperitoneal or intravenous immunization	12 Week
Cell Fusion	Select the mice with the best immune effect for cell fusion	13 Week
Cell Screening	Screening of positive cell lines by ELISA and other experimental methods	14 Week
Ascites preparation and purification	For WB antibody 1、 5 BALB/c mice will be immunized, and the best mouse will be chosen for fusion. 2、 1-3 positive clones will be delivered. 3、 1 clone will be used to make ascites followed by purification. For application-specific antibody (IHC, IF, IP, ChIP) 1、 10 mice will be immunized, and the best 2 mice will be chosen for fusion. 2、 1-3 positive clones will be delivered. 3、 3-5 clones will be used to make ascites followed by purification.	17 Week
Quality Appraisal	According to the customer’s downstream application, carry out corresponding quality inspection	21 Week
Subtyping	Subtyping of all positive cell lines and purified antibodies	22 Week

Remark

Host: BLAB/C mice;
Antigen requirements: concentration>1mg/ml, purity>85%, and the antigen solution does not contain organic solvents, tris, amino acids, etc.

Deliverables

3-5 strains of hybridoma cells
> 5 mg purified antibody
100 µL ascites stock solution
Project report, including a complete set of data on antigen production, hybridoma construction, screening, cloning and related testing

Mouse Monoclonal Antibody Service

Contant Us