E. coli Protein Service - NewEast Biosciences - GTPase, Oncogene and Bioactive Protein

E. coli Protein Service

E coli Protein Service

We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification.System

The advantages of E. coli expression systems include easy operation, low cost, high expression, as well as in-depth research on its genetic background and the diversity of vector strain selection, the use of E. coli to overexpress target genes is still the preferred method at present. However, the codon preferences of different species and the cytotoxicity and solubility of expressed products affect the expression of heterologous genes. With our rich experience, We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification.

Highlights	Details
High Success Rate	Success rate of the soluble protein expression provided using E. coli can be up to 95%.
Optional Expression Vectors	NewEast provides a variety of screened and reformed E. coli expression vectors to meet the specific requirements of intracellular and periplasmic; Space secretory expression including the pET series, the pBAD series, the pGEX series; The pCold series.
Optional Protein Tags	We use solubilized tags such as SUMO, Trx, GST, and NusA to improve; The hydrophilicity of protein in inclusion body expression.
Optional Expression Hosts	NewEast can reform and use more than 10 E. coli expression hosts; Including the standard expression strain, the protein expression enhanced strain, the toxic Protein expression strain, the ultra-low temperature strain for enhancing soluble protein expression, and the other expression strains, to meet the customers’ different requirements.
Large-scale Production	1-4,000 L per batch.
One-stop Service	From design and optimize gene, optimize protein expression conditions, purify protein and post processing, to managing of data and report.

Step	Description	Timeline
Plasmid Construction	Gene synthesis with codon optimization & subclone	2 Weeks
Protein Expression Test	Plasmid transformation Exploration of expression condition, expression analysis and identification (SDS-PAGE and/or WB) 200 mL amplification in optimum condition and purification test	3 Weeks
Large-Scale Fermentation & Purification	Scale up protein expression using optimized conditions Protein affinity purification (desired protein amounts and purity) Denaturation and renaturation of inclusion bodies (if required) Tag-free protein (if required), endotoxin removing (if required) Quality control	3-5 Weeks

E coli Protein Service

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