Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Ran-GTP
Cat. #: 26915 |
Size: 30 µL |
Gene Symbol: RAN |
Description: Anti-Ran-GTP Mouse Monoclonal Antibody |
Background: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. |
Immunogen: Recombinant full length protein of active Ran. |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Ran-GTP monoclonal antibody recognizes active Ran of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles |
Immunoprecipitation/Western blot: Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Ran-GTP monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody. |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase