Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Cat. #: 26911 |
Size: 30 µL |
Gene Symbol: RAB5 |
Description: Anti-Rab5-GTP Monoclonal Antibody |
Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. |
Immunogen: Recombinant full length protein of active Gao |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG1 |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot:
Rab5 Activation Assay.
Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rab5-GTP monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase