Size：30 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26902 |
| Size: 30 µL |
| Gene Symbol: Gna13 |
| Description: Anti-Gα13-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
| Immunogen: Recombinant full length active Gα13 protein |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004). |
Immunohistochemistry:
 Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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