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Arf6-GTP

26918

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Arf6-GTP

Cat. #:  26918
Size:  30 µL
Gene Symbol:  ARF6
Description:   Anti-Arf6-GTP Monoclonal Antibody
Background:   ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
Immunogen:   Recombinant full length protein of active Arf6.
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:    1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:    Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Arf6-GTP monoclonal antibody recognizes active Arf6 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  

Arf6 activation assay.

Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032).

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