Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26408 |
| Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| Description: Anti-IDH2(R172S) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172S IDH2 mutations do exist in diffusely infiltrative gliomas. |
| Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172S, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes R172S mutant, but not wild type IDH2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of recombinant IDH2(R172S) and wild type proteins.
Purified His-tagged IDH2(R172S) (lane 1) and corresponding wild type IDH2 protein (lane 2) were blotted with Anti-IDH2(R172S) monoclonal antibody (Cat. #26408).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172S) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH2(R172S) plasmid, pCDNA3-GFP-IDH2 (WT) plasmid, pCDNA3-GFP-IDH2 (R172G) plasmid, pCDNA3-GFP-IDH2 (R172K) plasmid, pCDNA3-GFP-IDH2 (R172W) plasmid or pCDNA3-GFP-IDH2 (R172H) plasmid, then fixed and stained with Anti-IDH2(R172S) monoclonal antibody (Cat. #26408)
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