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TP53(R282W)

26076

Size:100 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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TP53(R282W)
Cat. #:   26076
Gene Symbol:  TP53
Description:   Anti-TP53(R282W) Mouse Monoclonal Antibody
Background:   p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers.
Immunogen:   Recombinant p53(R282W) protein, human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-TP53(R282W) antibody recognizes TP53(R282W) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant TP53(R282W) and wild type p53 proteins. Purified His-tagged TP53(R282W) protein (lane 2, Cat.#10204) and wild type p53 protein (lane 1, Cat.#10202) were blotted with anti TP53(R282W) mouse monoclonal antibody (Cat. # 26076). Immunofluorescence:   Immunofluorescence of cells expressing TP53 proteins with anti-TP53 (R282W) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53(R282W) plasmid (right column), then fixed and stained with anti- TP53 (R282W) monoclonal antibody (Cat. # 26076).
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