Orai1-L1 pAb

21001

Size：100 μl Price：$ 219 Brand：NewEast Place of Origin：USA Immunogen：

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Orai1-L1 Polyclonal Antibody

Cat. #: 21001

Gene Symbol: Orai1

Description: Anti-Orai1-L1 Rabbit Polyclonal Antibody

Background: Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combined immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Immunogen: A peptide from the extracellular loop 1 region of human Orai1

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA:      1:1000-1:5000,
WB:          1:1000-1:2000,
IHC:          1:100-1:200

Concentration:

Host Species: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype: IgG

Purity: Immunogen affinity purified

Preservative: No

Constituents: 50 mM NaHCO₃-Glycine, 0.15M NaCl, pH7.4

Species Reactivity: Orai1-L1 antibody recognizes Orai1 from human and mouse. It may also recognize Orai2 and Orai3.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (lane 1) or presence (lane 2) of antigen peptide.

Immunohistochemistry:

Immunohistochemistry of endogenous Orai1 in NIH3T3 cells showing the plasma membrane staining.

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