Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
CTCF(K344E)
| Cat. #: 26324 |
| Gene Symbol: CTCF; MRD21 |
| Description: Anti-CTCF(K344E) Mouse Monoclonal Antibody |
| Background: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
| Immunogen: A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes K344E mutant, but not wild type CTCF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-CTCF(K344E) monoclonal antibody (Cat. #26324). Immunofluorescence: HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (right column), then fixed and stained with Anti-CTCF(K344E) monoclonal antibody (Cat. #26324). |
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