Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
IDH1(R132H)
| Cat. #: 26081 |
| Gene Symbol: IDH1 |
| Description: Anti-IDH1(R132H) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: Recombinant protein of human IDH1 which includes the mutation of R132H. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant IDH1(R132H) and wild type proteins.
Purified His-tagged IDH1(R132H) protein (Cat.#10199) and wild type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel).
Immunofluorescence:
Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1(R132H) plasmid (right column), then fixed and stained with Anti-IDH1(R132H) monoclonal antibody (Cat. #26081).
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