Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26477 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-RAS(G13V) Mouse Monoclonal Antibody |
| Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G13V mutant, but not wild type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wild type protein (lane 2), His-tagged Ras (G13S) protein (lane 3), His-tagged Ras (G13C) protein (lane 4), His-tagged Ras (G13R) protein (lane 5), His-tagged Ras (G13A) protein (lane 6) were blotted with Anti-RAS(G13V) monoclonal antibody (Cat. #26477).
Immunofluorescence:
Immunofluorescence of cells expressing Ras proteins with Anti-RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13R) plasmid, pCDNA3-GFP-Ras (G13A) plasmid or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with Anti-RAS(G13V) monoclonal antibody (Cat. #26477). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
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