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B-RAF mAb

26129

Size:100 μl Price:$ 239 Brand:NewEast Place of Origin:USA Immunogen:

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BRaf Antibody

Cat. #:  26129
Size:  100 µL
Gene Symbol:  B-Raf
Description:   Anti-B-RAF Mouse Monoclonal Antibody
Background:    B-Raf belongs to the TKL Ser/Thr protein kinase family, RAF subfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus, and may play a role in the postsynaptic responses of hippocampal neuron. B-Raf is located to nucleus and cytoplasm, and expressed in brain and testis. Immunogen: A synthetic peptide from the internal region of B-Raf, human origin.
Immunogen:   A synthetic peptide from the internal region of Braf (V600K), human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA 1:1000-1:5000
WB 1:100-1:1000
IHC 1:50-1:100
Concentration:    1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:    IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-B-Raf antibody recognizes B-Raf of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  image.png
Western blot analysis of recombinant B-Raf protein.

Purified His-tagged B-Raf protein (Cat. #10161) was blotted with anti-B-Raf monoclonal antibody (Cat. #26129).

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