| Cat. # : 36103 |
| Size : 100 µL |
| Gene Symbol: ACTB |
| Description: Anti-ß-actin Mouse Monoclonal Antibody |
| Background: Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. ß-actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas ß and ? actins, also known as cytoplasmic actins, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. |
| Immunogen: Synthetic peptide from the N-terminal of ß-actin |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions:
WB 1:10000-1:40000
IF 1:500-1:1000
IHC 1:100-1:500
|
| Concentration: 0.2 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Purity: Protein L purification from serum |
| Constituents: Tris buffer with 0.02% sodium azide and 50% glycerol pH 7.3 |
| Species Reactivity: Anti-ß actin antibody recognizes ß-actin of vertebrates and invertebrates |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis
Western blot analysis of β-actin in various cell lines or tissue using ?-actin antibody (10003-M01) at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1 hours. |
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Western blot analysis with Different dilutions
Different dilutions of WB were tested by whole lysate of mouse brain and 10003-M01 (β-actin antibody) antibody was used in this test. The amount of protein in each lane is 30ug. 10003-M01 antibody was incubated at room temperature for 1 hours. (Exposed for 2 seconds) |
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Place of Origin：USA
Immunogen：
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Immunofluorescent analysis of HEK-293 cells using 10003-M01 (β-actin antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
