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TIM3 (DM81) Rabbit mAb

28403

Size:100 μg Price:$ 445 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   28403
Product Name:   Anti-TIM3(DM81) Rabbit Monoclonal Antibody
Synonyms:   HAVCR2; TIM3; TIMD3; FLJ14428; KIM3
Description:    Anti-TIM3 antibody(DM81) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene belongs to the immunoglobulin superfamily; and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions; whereas; Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation; and inhibits Th1-mediated auto- and alloimmune responses; and promotes immunological tolerance.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human TIM3
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human TIM3 protein, mFc-His tagged protein 11158 can bind Rabbit anti-TIM3 monoclonal antibody ( clone: DM81) in a linear range of 0.32-40 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human TIM3  (B) were surface stained with Rabbit anti-TIM3 monoclonal antibody 1µg/ml ( clone: DM81) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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