CD22 (DM13) Rabbit mAb

28301

Size：100 μg Price：$ 445 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 28301

Product Name: Anti-CD22(DM13) Rabbit Monoclonal Antibody

Synonyms: SIGLEC-2; SIGLEC2

Description: Anti-CD22 antibody(DM13) Rabbit Monoclonal Antibody

Background: CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC2.

Applications: ELISA; Flow Cyt

Recommended Dilutions: Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human CD22

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti- CD22 monoclonal antibody 1µg/ml ( clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.	Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody ( clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.	Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.

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