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ICOSLG Rabbit pAb

213390

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S213390
Product Name:   Anti-ICOSLG Rabbit Polyclonal Antibody
Synonyms:   B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
UNIPROT ID:   O75144 (Gene Accession - NP_056074 )
Background:    Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.
Immunogen:    Synthetic peptide of human ICOSLG
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 213390(ICOSLG Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 213390(Anti-ICOSLG Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 and HUVEC cell lysates;
Primary antibody: 213390(ICOSLG Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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