Size：100 μg
Price：$ 389
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat.#: 12253 |
| Product Name: Human CD3D Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CD3-DELTA;IMD19;T3D and IMD18;T3E;TCRE |
| Target: CD3E |
| UNIPROT ID: P04234;P07766 |
| Description: Recombinant Human CD3D Protein with C-terminal 6xHis tag and Human CD3E Protein with C-terminal human Fc tag |
| Background: T-cell surface glycoprotein CD3 delta and CD3 epsilon chain, also known as CD3D and CD3E or CD3DandCD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation. |
| Molecular Characterization: CD3D(Phe22-Ala105) 6×His tag and CD3E(Asp23-Asp126) hFc(Glu99-Ala330) |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD3D&CD3E Heterodimer Protein, His Tag & hFc Tag on SDS-PAGE under reducing condition. |
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